gradient gel electrophoresis dgge  (Bio-Rad)

Journal: Microorganisms

Article Title: Dietary Effects of a Short-Term Administration of Microalgae Blend on Growth Performance, Tissue Fatty Acids, and Predominant Intestinal Microbiota in Sparus aurata .

doi: 10.3390/microorganisms11020463

Figure Lengend Snippet: Figure 1. Clustering based on pairwise similarity index of DGGE patterns obtained from intestinal samples of juvenile S. aurata fed experimental diets for 30 days. Codes are: MB-5: 5% microalgae blend meal inclusion; MB-15: 15% microalgae blend meal inclusion; MB-25: 25% microalgae blend meal inclusion.

Article Snippet: PCR products were separated by denaturing gradient gel electrophoresis (DGGE) in a Dcode TM system (Bio-Rad Laboratories, United States), according to Rico et al. [27].

Techniques:

Journal: Microorganisms

Article Title: Dietary Effects of a Short-Term Administration of Microalgae Blend on Growth Performance, Tissue Fatty Acids, and Predominant Intestinal Microbiota in Sparus aurata .

doi: 10.3390/microorganisms11020463

Figure Lengend Snippet: Figure 2. Pareto–Lorenz distribution curves based on PCR-DGGE patterns from intestinal samples) of juvenile S. aurata fed experimental diets for 30 days at day 0, 7, 15, and 30 ((a–d), respectively). Codes are: MB-5: 5% microalgae blend meal inclusion; MB-15: 15% microalgae blend meal inclusion; MB-25: 25% microalgae blend meal inclusion.

Article Snippet: PCR products were separated by denaturing gradient gel electrophoresis (DGGE) in a Dcode TM system (Bio-Rad Laboratories, United States), according to Rico et al. [27].

Techniques:

Journal: Scientific Reports

Article Title: The effect of salinity on soil chemical characteristics, enzyme activity and bacterial community composition in rice rhizospheres in Northeastern Thailand

doi: 10.1038/s41598-022-24902-2

Figure Lengend Snippet: Denaturing gradient gel electrophoresis (DGGE) profiles of nested PCR products amplified from bacteria in the rhizosphere samples of genotype ‘RD 6’ rice planted in various saline fields. Three profiles of DGGE from each field are shown such as Ban Wa: lanes 1–3, Ban Pai: lanes 4–6, Ban Thum: lanes 7–9, and Phra Yuen: lanes 10–12. The DNA bands selected for further sequencing analysis are denoted by the letter a – n.

Article Snippet: PCR amplification was carried out in a FlexCycler2 thermal cycler (Analytik Jena, Germany) using the program as follows: pre-denaturation at 94 °C for 10 min, followed by 34 cycles at 94 °C for 30 s, annealing at 55 °C for 30 s, elongation at 72 °C for 45 s, followed by a final extension at 72 °C for 7 min. DGGE analysis was performed using a TV400-DGGE denaturing gradient gel electrophoresis system (Scie-Plas, UK).

Techniques: Denaturing Gradient Gel Electrophoresis, Nested PCR, Amplification, Sequencing

Journal: Scientific Reports

Article Title: The effect of salinity on soil chemical characteristics, enzyme activity and bacterial community composition in rice rhizospheres in Northeastern Thailand

doi: 10.1038/s41598-022-24902-2

Figure Lengend Snippet: The unrooted phylogenetic tree of 16S rRNA gene sequence with the selective bands presented in the DGGE profile. The tree was conducted using the Neighbor-Joining method. The percentage of bootstrapping values (1000 replicates) are presented on each clade.

Article Snippet: PCR amplification was carried out in a FlexCycler2 thermal cycler (Analytik Jena, Germany) using the program as follows: pre-denaturation at 94 °C for 10 min, followed by 34 cycles at 94 °C for 30 s, annealing at 55 °C for 30 s, elongation at 72 °C for 45 s, followed by a final extension at 72 °C for 7 min. DGGE analysis was performed using a TV400-DGGE denaturing gradient gel electrophoresis system (Scie-Plas, UK).

Techniques: Sequencing

Journal: Mycorrhiza

Article Title: Possible role of arbuscular mycorrhizal fungi and associated bacteria in the recruitment of endophytic bacterial communities by plant roots

doi: 10.1007/s00572-021-01040-7

Figure Lengend Snippet: Bacterial communities closely associated with spores, sporocarps, extraradical mycelium and intraradical propagules of AMF species and isolates from different geographic locations

Article Snippet: Culture-independent techniques, PCR-denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq sequencing of the 16S rDNA of root endophytic bacterial communities confirmed the selectivity of genotypes in durum wheat, as different cultivars hosted significantly different bacterial communities in their root tissues (Agnolucci et al. ).

Techniques: Isolation, Sequencing, Clone Assay